Title

The Impact of Raman Spectroscopy on Concentration and Purity DNA Amelogenin from Dental Samples.

Authors

Riki Kristanto, I Ketut Junitha, Ir. Hery Suyanto, Ir. Made Pharmawati, Raouf Fetni

Abstract

Background: Analysis Laser by Raman spectroscopy is widely used to sex estimation of dental samples. Generally, sex estimation of dental samples can be identified by DNA amelogenin examination, but there is a lack of research on the impact of laser administration on concentration results and DNA purity in sex identification from dental samples. Aim: To determine the impact of laser application from Raman spectroscopy on the concentration and purity of DNA amelogenin for sex estimation. Methods: The tooth samples came from the same individual, with 1 right tooth and 1 left tooth. Samples were obtained from tooth extraction for orthodontic pretreatment. The tooth sample was cut horizontally on the occlusal surface to the tooth apex, then laser light was applied with a wavelength of 100-3400 cm-1, on the enamel, dentin, and pulp chamber layers. The layers of teeth that had been shot with a laser were powder on each layer and DNA amelogenin analysis was performed. The total is 6 samples. Results: Examination of the concentration and purity of amelogenin DNA showed values between 1.57 – 1.87 µn, but the results of PCR analysis showed 1 of 6 did not have the same match in gender identification. Conclusion: This preliminary study showed that there was had impact of laser of Raman spectroscopy on the concentration and purity of DNA amelogenin from dental samples, and also there was an impact on gender determination.

Keywords

Purity, Laser, Raman Spectrocopy, DNA Amelogenin, Dental Sample.